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24-well cluster dishes  (Greiner Bio)


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    Structured Review

    Greiner Bio 24-well cluster dishes
    24 Well Cluster Dishes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/24+well+cluster+dishes/24+well+cluster+dishes/pm33560544-56-22-25
    Average 90 stars, based on 1 article reviews
    24-well cluster dishes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage
    Article Snippet: .. For the determination of mRNA expression, dispersed luteal cells were seeded at 2.0 × 10 5 viable cells per m l in 24-well cluster dishes (Greiner Bio-One, Frickenhausen, Germany; No. 662160), while 48-well cluster dishes (Thermo Fischer Scientific, Rochester, NY, U.S.A.; No. 130187) were used for the determination of P4 production. ..

    Article Title: Hypoxia Promotes Progesterone Synthesis During Luteinization in Bovine Granulosa Cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passage was done using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The granulosa cells were seeded at a concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/ well; Greiner Bio-One; 662160) for determination of P4 production, in 24-well cluster dishes (1.0 ml/ well; Greiner Bio-One; 677180) for determination of gene expression and in 75 cm 2 culture flasks (20 ml/ flask; Greiner Bio-One; 658175) for determination of protein expression. .. To prepare luteinizing and non-luteinizing granulosa cells, the culture medium was replaced with fresh medium containing 0.1% BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132) and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries, Osaka, Japan; 031–12061), and the cells were then incubated under a normal culture atmosphere (20% O 2 , 5% CO 2 , 75% N 2 ) without or with insulin (2 μg/ml; Sigma-Aldrich; I4011), forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70–0501-05) or insulin (2 μg/ml) in combination with forskolin (10 μM) for 24 h. The concentration of insulin and forskolin was selected based on a previous report [ ].

    Article Title: FAS/FASL-mediated cell death in the bovine endometrium.
    Article Snippet: In many mammals, endometrial cells are remodeled by apoptosis and cell proliferation throughout the estrous cycle.. Although apoptosis is known to be induced by various factors involving two major apoptotic pathways (the death receptorand mitochondria-mediated pathways), how it is regulated in the bovine endometrium is unclear.. We examined (1) the cyclic expressions of apoptosis-related factors, FAS, DcR3, BCL2 and BAX, in the bovine endometrium and (2) the effect of death ligands on the viability of, and FAS mRNA expression in, cultured bovine endometrial epithelial and stromal cells.

    Article Title: Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passaging was conducted using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The GCs were seeded at the concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/well; Greiner Bio-One; 662160) for quantification of P4 production, in 96-well cluster dishes (0.1 ml/well; Iwaki, Chiba, Japan; 3860-096) for the cell viability assay, in 24-well cluster dishes (1.0 ml/well; Greiner Bio-One; 677180) for determination of gene expression, and in 75 cm 2 culture flasks (20 ml/flask; Greiner Bio-One; 658175) for analysis of protein expression. .. To prepare luteinizing and non-luteinizing GCs, the culture medium was replaced with a fresh medium containing 0.1% of BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132), and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries Osaka, Japan; 031-12061), and the cells were then incubated in a normal culture atmosphere (20% O 2 , 5% CO 2 , and 75% N 2 ) with or without insulin (2 μg/ml; Sigma-Aldrich; I4011) in the medium in combination with forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70-0501-05) for 24 h. Insulin and insulin-like growth factor I (IGF-I) are known to stimulate proliferation of (and P4 production in) GCs [ , , , , ].

    Article Title: Effect of elevated temperatures on bovine corpus luteum function: expression of heat-shock protein 70, cell viability and production of progesterone and prostaglandins by cultured luteal cells
    Article Snippet: Summer heat stress lowers fertility in cattle in hot environments by influencing oocyte quality, follicular activity and progesterone (P4) level in blood plasma.. However, the mechanisms by which elevated temperature influences corpus luteum function remain unclear.. Elevated temperature has generally been known to upregulate the gene expression of heat-shock protein (HSP) 70 in a variety of cell types.

    Article Title: Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells
    Article Snippet: .. To measure P4 production and mRNA expression, dispersed luteal cells were seeded at 4.0 × 10 5 viable cells per ml in 24-well cluster dishes (Greiner Bio-One, Frickenhausen, Germany; No. 662160). ..

    Cell Culture:

    Article Title: Genomic and non-genomic effects of progesterone on prostaglandin (PG) F2α and PGE2 production in the bovine endometrium
    Article Snippet: Cell culture The final pellets of the epithelial and stromal cells were separately resuspended in culture medium (DMEM/Ham’s F-12, 1 : 1 (v/v); Invitrogen, Carlsbad, CA, USA) supplemented with 10% (v/v) bovine serum (Invitrogen), 20mgmL 1 gentamicin (Sigma-Aldrich) and 2mgmL 1 amphotericin B (SigmaAldrich) (Skarzynski et al. 2000). .. Epithelial cells were seeded at a density of 1 10 viable cells mL 1 in culture flasks (Greiner Bio-One, Frickenhausen, Germany) and stromal cells were seeded at a density of 1 10 viable cells mL 1 in 4-well cluster dishes (Thermo Fisher Scientific, Yokohama, Japan) and 24-well cluster dishes (Greiner Bio-One) and were cultured at 38.58C in a humidified atmosphere of 5% CO2 in air. ..

    Article Title: Evidence for a PGF2α auto-amplification system in the endometrium in mares
    Article Snippet: .. 173 The dispersed epithelial and stromal cells were seeded separately at a density of 174 1×10 5 viable cells/ml in 24-well cluster dishes (662160; Greiner Bio-One, 175 Frickenhausen, Germany) and cultured at 38.0°C in a humidified atmosphere of 5% 176 CO2 in air. ..

    Concentration Assay:

    Article Title: Hypoxia Promotes Progesterone Synthesis During Luteinization in Bovine Granulosa Cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passage was done using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The granulosa cells were seeded at a concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/ well; Greiner Bio-One; 662160) for determination of P4 production, in 24-well cluster dishes (1.0 ml/ well; Greiner Bio-One; 677180) for determination of gene expression and in 75 cm 2 culture flasks (20 ml/ flask; Greiner Bio-One; 658175) for determination of protein expression. .. To prepare luteinizing and non-luteinizing granulosa cells, the culture medium was replaced with fresh medium containing 0.1% BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132) and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries, Osaka, Japan; 031–12061), and the cells were then incubated under a normal culture atmosphere (20% O 2 , 5% CO 2 , 75% N 2 ) without or with insulin (2 μg/ml; Sigma-Aldrich; I4011), forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70–0501-05) or insulin (2 μg/ml) in combination with forskolin (10 μM) for 24 h. The concentration of insulin and forskolin was selected based on a previous report [ ].

    Article Title: Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passaging was conducted using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The GCs were seeded at the concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/well; Greiner Bio-One; 662160) for quantification of P4 production, in 96-well cluster dishes (0.1 ml/well; Iwaki, Chiba, Japan; 3860-096) for the cell viability assay, in 24-well cluster dishes (1.0 ml/well; Greiner Bio-One; 677180) for determination of gene expression, and in 75 cm 2 culture flasks (20 ml/flask; Greiner Bio-One; 658175) for analysis of protein expression. .. To prepare luteinizing and non-luteinizing GCs, the culture medium was replaced with a fresh medium containing 0.1% of BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132), and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries Osaka, Japan; 031-12061), and the cells were then incubated in a normal culture atmosphere (20% O 2 , 5% CO 2 , and 75% N 2 ) with or without insulin (2 μg/ml; Sigma-Aldrich; I4011) in the medium in combination with forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70-0501-05) for 24 h. Insulin and insulin-like growth factor I (IGF-I) are known to stimulate proliferation of (and P4 production in) GCs [ , , , , ].

    Gene Expression:

    Article Title: Hypoxia Promotes Progesterone Synthesis During Luteinization in Bovine Granulosa Cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passage was done using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The granulosa cells were seeded at a concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/ well; Greiner Bio-One; 662160) for determination of P4 production, in 24-well cluster dishes (1.0 ml/ well; Greiner Bio-One; 677180) for determination of gene expression and in 75 cm 2 culture flasks (20 ml/ flask; Greiner Bio-One; 658175) for determination of protein expression. .. To prepare luteinizing and non-luteinizing granulosa cells, the culture medium was replaced with fresh medium containing 0.1% BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132) and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries, Osaka, Japan; 031–12061), and the cells were then incubated under a normal culture atmosphere (20% O 2 , 5% CO 2 , 75% N 2 ) without or with insulin (2 μg/ml; Sigma-Aldrich; I4011), forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70–0501-05) or insulin (2 μg/ml) in combination with forskolin (10 μM) for 24 h. The concentration of insulin and forskolin was selected based on a previous report [ ].

    Article Title: Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passaging was conducted using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The GCs were seeded at the concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/well; Greiner Bio-One; 662160) for quantification of P4 production, in 96-well cluster dishes (0.1 ml/well; Iwaki, Chiba, Japan; 3860-096) for the cell viability assay, in 24-well cluster dishes (1.0 ml/well; Greiner Bio-One; 677180) for determination of gene expression, and in 75 cm 2 culture flasks (20 ml/flask; Greiner Bio-One; 658175) for analysis of protein expression. .. To prepare luteinizing and non-luteinizing GCs, the culture medium was replaced with a fresh medium containing 0.1% of BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132), and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries Osaka, Japan; 031-12061), and the cells were then incubated in a normal culture atmosphere (20% O 2 , 5% CO 2 , and 75% N 2 ) with or without insulin (2 μg/ml; Sigma-Aldrich; I4011) in the medium in combination with forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70-0501-05) for 24 h. Insulin and insulin-like growth factor I (IGF-I) are known to stimulate proliferation of (and P4 production in) GCs [ , , , , ].

    Viability Assay:

    Article Title: Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells
    Article Snippet: When the cultured cells reached 80–90% confluence, cell passaging was conducted using 0.1% bovine trypsin (Sigma-Aldrich; T92012) and sterile phosphate-buffered saline (PBS; Nissui Pharmaceutical, Tokyo, Japan; 05913). .. The GCs were seeded at the concentration of 2.0 × 10 5 viable cells per 1 ml in 48-well cluster dishes (0.5 ml/well; Greiner Bio-One; 662160) for quantification of P4 production, in 96-well cluster dishes (0.1 ml/well; Iwaki, Chiba, Japan; 3860-096) for the cell viability assay, in 24-well cluster dishes (1.0 ml/well; Greiner Bio-One; 677180) for determination of gene expression, and in 75 cm 2 culture flasks (20 ml/flask; Greiner Bio-One; 658175) for analysis of protein expression. .. To prepare luteinizing and non-luteinizing GCs, the culture medium was replaced with a fresh medium containing 0.1% of BSA, 5 ng/ml sodium selenite (Sigma-Aldrich; S5261), 5 μg/ml transferrin (Sigma-Aldrich; T4132), and 0.5 mM ascorbic acid (Wako-Pure Chemical Industries Osaka, Japan; 031-12061), and the cells were then incubated in a normal culture atmosphere (20% O 2 , 5% CO 2 , and 75% N 2 ) with or without insulin (2 μg/ml; Sigma-Aldrich; I4011) in the medium in combination with forskolin (10 μM; Research Biochemicals International, Natick, MA, USA; 70-0501-05) for 24 h. Insulin and insulin-like growth factor I (IGF-I) are known to stimulate proliferation of (and P4 production in) GCs [ , , , , ].



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